This study explored how singers adapt their vocal technique in response to different performance environments recreated through virtual reality, with implications for vocal practice. Fifteen soprano singers performed two songs in three virtual venues, each carefully designed using immersive audio and 360-degree visual imagery captured from real-world spaces in Bangkok, Thailand: a dry studio (C300) and a purpose-built concert hall (Sangita Vadhana Hall), both located at the Princess Galyani Vadhana Institute of Music, and a large reverberant church (Holy Rosary Church). Subtle yet consistent changes were observed—larger, more reverberant venues with grander visuals led to brighter tone, slower and less pronounced vibrato, and reduced pitch accuracy, while the concert hall encouraged louder singing with greater tremolo. These adaptations, not always aligned with the singers’ conscious impressions, suggest that both acoustic and visual cues may shape performance subconsciously. The findings highlight the potential of virtual reality environments as a practical training tool, enabling singers to refine their technique and adapt to diverse venues before live performance.